Authors : He YaLin, Zhu Ling, Feng Xiaoke, Zhao Ling, Fu Mengjin, Mei Miao, Wu Yunfei, Xu Zhi- Wen and Guo Wanzhu
Abstract: According to the published genomic sequence of TTSuV1 (GenBank Accession No. AB076001.1), the pairs of specific PCR/nested-PCR primers were designed to amplify the full length of TTSuV1 complete genome from blood serum samples in Sichuan province. The amplified fragments were cloned, sequenced and named as TTSuV1-SC1 and TTSuV1-SC2, respectively. The sequencing results showed that the complete genome of them were 2852 bp and 2917 bp in length. And then, the correct target sequences gained were deposited in GenBank and the GenBank accession number JF694116 and JF694117 were generated. Homology analysis of TTSuV1-SC1, TTSuV1-SC2 and reference strain sequences retrieved in GenBank indicated that the complete genome of TTSuV1-SC1 and TTSuV1-SC2 from Sichuan isolated strains shared 81-98 and 82-99% homology, respectively with those reference sequences. The phylogenetic tree analysis indicated that the closest genetic relationship of TTSuV1-SC1 and TTSuV1-SC2 isolated strains were TTV1Bj10 strain HM633251.1 and TTVSH0822/2008 strain (GU450331.1). The study would lay a biological foundation to understand the epidemiology, genetic variation of the virus.
He YaLin, Zhu Ling, Feng Xiaoke, Zhao Ling, Fu Mengjin, Mei Miao, Wu Yunfei, Xu Zhi- Wen and Guo Wanzhu, 2012. Cloning and Sequence Analysis of the Complete Genome of Porcine Torque Teno Sus Virus Type 1. Journal of Animal and Veterinary Advances, 11: 3755-3760.