Abstract: The normal response on the airway epithelial lining given injury comprises migration, proliferation and redifferentiation. Researchers reported here a novel brushing technique for inflicting injury on the tracheal airway, termed blinded brushing technique using rabbits as a model. Rabbits were categorised as either treated with blinded-tracheal brushing or untreated as to serve as control for normal tracheal epithelium structure. Researchers subsequently euthanized all rabbits in different time points (ranges from for 30 min and 1, 6, 12 and 24 h) post-infliction in order to examine the effect of the brushing to the tracheal epithelium structure. The results demonstrated that this technique was successfully removed the intact epithelium layer and its basement membrane without prior knowledge of location and position of the target site on the tracheal epithelium. The length of the induced-injuries were measured between the edges of the remaining epithelium bordering the lesion and the length of the injured areas were gradually decreased over the time points as compared to 30 min following injury. The decreases of the length of the injuries indicate that regeneration process was activated as to restore the normal epithelium layer. As conclusion, researchers had successfully developed a more practical and time-efficient brushing technique that could be very useful technique as to provoke cellular and molecular activations during airway regeneration and repair in respond to injury.