Authors : Junwei Cao, Libing Ma, Hua Song, Huanmin Zhou and Yanru Zhang
Abstract: Mitochondria is a kind of important organelle for producing and supplying energy in eukaryotes. The interspecies somatic nuclear transfer could result in coexisting of nuclear donor and ooplasmic recipient mitochondria in reconstructed embryos. In the present study, after nuclear donor and/or ooplasmic recipient mitochondria damaged by photoirradiating rhodamine-123, examination of the goat-sheep interspecies somatic nuclear transfer embryos early development ability in vitro was performed. The result shows that sheep ooplasmic recipient mitochondria damaged could result in reconstructed embryos development ability declined in 2-cell and morulae/blastocyst stage but does not affect 2-cells stage to 8-cells stage development; goat nuclear donor cells mitochondria damaged can not affect reconstructed embryos development ability before morulae/blastocyst stage; after both nuclear donor and ooplasmic recipient mitochondria are damaged, the reconstructed embryos development pattern is same as that of only ooplasmic recipient mitochondrial damaged. The result indicated that before interspecies somatic nuclear transfer reconstructed embryo development to morulae/blastocyst stage, ooplasmic recipient mitochondrial is dominant over nuclear donor mitochondrial in affecting reconstructed embryos development ability.
Junwei Cao, Libing Ma, Hua Song, Huanmin Zhou and Yanru Zhang, 2012. Effect of Mitochondrial Dysfunction in Nuclear Donor and Ooplasmic Recipient on Early Development of Goat-Sheep Interspecies Somatic Nuclear Transfer Embryos. Journal of Animal and Veterinary Advances, 11: 4079-4085.