Authors : Tuerxun Paxia, Gao-Feng Wu, Xiao-Liang Pan and Zi-Rong Xu
Abstract: In this research, researchers assessed the gossypol induced toxicity on Xinjiang Fine-Wool sheep Leydig cell to get a better understand of the reproduction toxicity of gossypol on the male ruminant. The Leydig cells with high purity were separated successfully from a testicle of 3 months old Fine-Wool sheep through a method of Percoll Density Gradient Centrifugation. Cytotoxicity assays indicated mitochondrial is sensitive to gossypol induced toxicity. Hoechst staining and DNA-FCM with PI staining revealed gossypol mainly causes cell apoptosis of the Leydig cell. Cell cycle analysis by FCM indicated cell cycle arrest on G0/G1 phase. Leydig cell is the main site of testosterone biosynthesis and testosterone is the critical steroid hormone which determines the initiation and maintenance of spermatogenesis as well as expression of the male phenotype. Thus, cytotoxicity effects of gossypol on the Leydig cell revealed by this research indicated that the reduction of spermatogenesis induced by gossypol in male ruminant at least partially associated with the cytotoxicity effects of gossypol on the Leydig cell.
Tuerxun Paxia, Gao-Feng Wu, Xiao-Liang Pan and Zi-Rong Xu, 2012. In vitro Assessment of Gossypol Induced Toxicity in Xinjiang Fine-Wool Sheep Leydig Cell. Journal of Animal and Veterinary Advances, 11: 4102-4107.