Journal of Animal and Veterinary Advances

Year: 2012
Volume: 11
Issue: 23
Page No. 4435 - 4440

The Effect of Helicobacter pylori on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid in vitro

Authors : J.G. Chen, A.X. Liang, L. Han, J.J. Xiong, A.Z. Guo and L.G. Yang

Abstract: To investigate the effect of Helicobacter pylori on apoptosis of the GES-1 transfected pEGFP-N1-UreB eukaryotic expression plasmid in vitro. The Hp-UreB gene was amplified by PCR and cloned into eukaryotic expression plasmid pEGFP-NI to construct recombinant plasmid pEGFP-NI-UreB and transfected into GES-1 cells and screened by G418. GES-1 cells transfected into plasmid pEGFP-NI-UreB were co-cultured with H. pylori SS1 and its apoptosis was quantified by flow cytometry using Annexin V-APC and 7-AAD. Changes in cell morphology were observed by transmission electron microscope. The recombinant pEGFP-N1-UreB was constructed and stable transfected into GES-1 cells. The apoptotic rates of cells transfected into pEGFP-N1-UreB plasmids were significantly lower than those of control cells (p<0.05).

How to cite this article:

J.G. Chen, A.X. Liang, L. Han, J.J. Xiong, A.Z. Guo and L.G. Yang, 2012. The Effect of Helicobacter pylori on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid in vitro. Journal of Animal and Veterinary Advances, 11: 4435-4440.

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