Authors : Ping Rui, Zengjun Ma , QiuYue Wang , Hai Fang , JianHua Wu , ZhiXin Fu , Qing Hui Jia and Xuexia Wen
Abstract: To detect the relationship between fluoroquinolone resistance and gyrA, parC gene mutation in Streptococcus suis type 2. The MIC of 14 strains of Streptococcus suis type 2 were determined by microdilution. The genes encoding the Quinolone-Resistance Determining Region (QRDRs) of parC and gyrA in fluoroquinolone-susceptible and resistant Streptococcus suis clinical isolates were identified and sequenced. Ser to Phe and Arg to Leu mutation at position 79 and 87 of the parC gene was detected in fluoroquinolone resistance SS2 and Arg to Ser or Ser to Arg mutations at the position 66 or 81 of gyrA gene were detected in 2 highly resistance strains; no amino acid changes in gyrA or parC were detected for 7 fluoroquinolone-susceptible strains; the mutations in both genes were found in the strains with MIC of fluoroquinolone >32 μg mL-1. Mutations in parC gene may result in low level resistance against fluoroquinolone and mutations in both gyrA and parC genes result in high level resistance.
Ping Rui, Zengjun Ma , QiuYue Wang , Hai Fang , JianHua Wu , ZhiXin Fu , Qing Hui Jia and Xuexia Wen , 2012. Relationship Between Fluoroquinolone Resistance and gyrA, parC Gene in Streptococcus suis Type 2 Isolates in Hebei Province China. Journal of Animal and Veterinary Advances, 11: 719-722.