Authors : Jin Wenjie, Zheng Zhiming, Zhang Yongzhi, Wang Qianqian, Qin Aijian, Shao Hongxia and Qian Kun
Abstract: Two hundred and sixteen Avian Pathogenic E. coli (APEC) isolates were used for the determination of drug susceptibility to β-lactam and the detection of β-lactam drug resistance-related genes OXA-1, OXA-5, OXA-31 using circular Disk Diffusion Method and PCR. Results showed that drug sensitivity rates among isolates were 190/216, 191/216, 162/216 to cefotaxime, ceftriaxone and ampicillin/sulbactam, respecively. The amplified gene fragments analysis of β-lactam drug resistance-related gene sequence of isolates showed 97%, homology with reference sequences from the GenBank. The OXA-1 and OXA-31 positive rates were only 7.4 and 10.6% but the OXA-5 positive rate was 30.6%. Seventy five strains carried only one kind of resistance genes, twelve strains carried two kinds of resistance genes and two strains carried all the three kinds of resistance genes. The integrated results of resistance gene detection and drug sensitivity test showed that the strains isolated in APEC on the rate of drug resistance testing and three resistance gene detection rate compared to the rate of aminoglycoside resistance and their drug resistance gene and the detection rate is not high but the growing development of bacterial resistance this is the alert source of bacteria in the prevention and treatment of poultry should pay close attention to rational drug use. In this study, the source for the understanding of avian bacteria β-lactam drug resistance mechanisms as well as generate a broader range of further drug resistance genes to provide a reference to epidemiological studies.
Jin Wenjie, Zheng Zhiming, Zhang Yongzhi, Wang Qianqian, Qin Aijian, Shao Hongxia and Qian Kun, 2013. Distribution of Three OXA-Type β-Lactamase Encoded Genes in Avian Pathogenic E. coli Isolated in China. Journal of Animal and Veterinary Advances, 12: 859-862.