Authors : Nian Fuzhao, Zhang Yonghui, Su Xiangyun, Zou Juqiu and Zhao Leifeng
Abstract: Flavonols have significant health benefits for animals and humans. Flavonol synthase has been characterized to be required for flavonol biosynthesis. The complete coding sequence of tobacco Flavonol synthase gene was amplified by RT-PCR. The complete coding sequence of tobacco Flavonol synthase gene was 951 bp which encodes a protein of 316 amino acids. Sequence analysis revealed that the flavonol synthase of tobacco shares high homology with the flavonol synthase of Lycopersicon esculentum (69%), castor bean (64%), cucumber (58%) and chickpea (57%). Phylogenetic tree analysis revealed that the tobacco Flavonol synthase gene has a closer genetic relationship with that of Lycopersicon esculentum. Expression profile was studied and the results indicated that tobacco Flavonol synthase gene was highly expressed in in leaf and flower. These results established the primary foundation of utilization of tobacco flavonols in the future.
Nian Fuzhao, Zhang Yonghui, Su Xiangyun, Zou Juqiu and Zhao Leifeng, 2013. Molecular Characterization and Expression Pattern of a Novel Flavonol Synthase gene. Journal of Animal and Veterinary Advances, 12: 937-941.