Authors : Lu Bai, Jideng Ma, Yue Wang, Zhengjian Zhao, Xuewei Li, Anan Jiang and Lin Bai
Abstract: Blood plays an important role in many biological processes and circulating microRNAs (miRNAs) in the blood have been reported to be biomarkers for various physiological and pathological states. However, few studies have examined porcine blood miRNAs, especially arterial blood miRNAs. Here, researchers sequenced the microRNAomes of porcine arterial and venous blood samples in which we identified 277 and 294 known porcine miRNAs, respectively. In both blood samples, highly expressed miRNAs were implicated in erythropoiesis and angiogenesis. A total of 57 miRNAs showed significantly different expression levels (p<0.01) between arterial and venous blood of which seven were enriched in arterial blood and five were enriched in venous blood by more than a 1.5 fold change. Interestingly, functional enrichment analysis of the genes predicted to be targeted by the enriched miRNAs indicated that arterial blood was mainly associated with the immune response and venous blood was primarily associated with apoptosis and the hypoxia response. The results suggest that the 12 miRNAs with differential expression should be considered as biomarkers for physiological differences between arterial and venous blood and that they may help promote the further molecular diagnosis of blood.
Lu Bai, Jideng Ma, Yue Wang, Zhengjian Zhao, Xuewei Li, Anan Jiang and Lin Bai, 2014. MicroRNAomes of Porcine Arterial and Venous Blood. Journal of Animal and Veterinary Advances, 13: 21-27.