Authors : Jie-Lin Duan, Shao-Ping He, Guan Yang, Jie Yin, Wen-Kai Ren, Jian-Wen Deng, Yu Gong, Feng-Ming Chen, Tie-Jun Li and Xing-Guo Huang
Abstract: Probiotics, regarded as live microorganisms, play important roles in regulating intestinal inflammatory responses and innate immunity. Although, intestinal immunmodulation of probiotics have been enormously conducted into data, the effects of Lact. delbrueckii on jejunum innate immunity are rarely reported. This study aimed to investigate the effects of Lact. delbrueckii on serum profiles, jejunum inflammatory cytokines and innate immune-related genes expression in mouse. A total of 32 Kunming male mice were randomly divided into four groups and each group were orally administrated with saline normal, living Lact. delbrueckii, heat-killed Lact. delbrueckii and Spent Culture Supernatant (SCS). Serum profiles (IL-2, IgG) and jejunum immune-related gene expression were detected including inflammatory cytokines (INF-γ, TNF-α, IL-17, IL-13) peneth cell-sepcific gene (cryptdin 1, 4, 5, lysozyme C, CRS-1C, CRS-4C and sPLA2) and goblet cell-specific gene (mucin-2 and mucin-4). Compared with control group, orally administrated SCS significantly decreased (p<0.05) ADG of mice. Orally administrated living and heat-killed Lact. delbrueckii group significantly increased (p<0.05) serum IL-2 level and while SCS remarkably decreased (p<0.05) serum IgG level. Meanwhile, oral administration of living Lact. delbrueckii significantly enhanced (p<0.05) jejunum inflammatory cytokines (INF-γ, TNF-α, IL-17) expression. SCS significantly increased (p<0.05) peneth cell-sepcific gene (cryptdin 1, 4, 5 and sPLA2) gene expression. In conclusion, oral administration of Lact. delbrueckii affects serum profiles and jejunum innate immune-related gene expression and thereby modulates jejunum innate immunity.
Jie-Lin Duan, Shao-Ping He, Guan Yang, Jie Yin, Wen-Kai Ren, Jian-Wen Deng, Yu Gong, Feng-Ming Chen, Tie-Jun Li and Xing-Guo Huang, 2014. Effect of Lactobacillus delbrueckii on Jejunum Innate Immune-Related Gene Expression in Mice. Journal of Animal and Veterinary Advances, 13: 989-997.