Abstract: Semen preservation is a critical procedure to guarantee that good quality semen is adequate for advanced assisted reproduction. The present study aimed to evaluate viability of Nguni bull spermatozoa diluted with modified Ham=s F10 culture medium stored at different storage conditions (5, 12, 17°C and controlled room temperature 24°C) for 72 h. Following microscopic evaluations, uncontaminated semen samples with progressive motility >70% were pooled before being aliquoted randomly into four test tubes of modified Ham=s F10. Diluted samples were distributed unsystematically to each of the four temperature conditions (5, 12, 17 and 24°C) and stored for 72 h. Computer aided sperm analysis was used to evaluate sperm motility, DNA fragmentation and viability. The highest spermatozoa motility rate (86.5%) and viability (26.5%) was observed with 24°C as compared to the other three temperatures 17°C (69.5; 8.0%), 12°C (50.3; 2.5%) and 5°C (35.1; 0.5%), respectively for 72 h. The overall sperm viability rate of the storage conditions 24, 17, 12 and 5°C<30% after 72 h. There was no significant difference in sperm DNA fragmentation amongst the four storage conditions (p<0.01). In conclusion, motility and viability rate of Nguni spermatozoa extended with modified Ham=s F10 culture medium decreased noticeably regardless of the storage temperature condition.
A.M. Raseona and D.M. Barry, 2018. Extension of Fresh Bull Semen in Ham's F10 Stored at Different Storage Conditions for 72 Hours. Journal of Animal and Veterinary Advances, 17: 133-138.