Abstract: Chemical demulsification process is the most widely applied method of treating water-in-crude oil emulsions and involves the use of chemical additives to accelerate the emulsion breaking process. Crude oil emulsions are stabilized by asphaltenes which are colloidally dispersed in the crude oil. Asphaltenes consist mainly of polar heterocompounds and is known that they decrease the interfacial tension between oil and water and form stable interfacial films. This research investigates the performance of demulsifiers in relation to whole asphaltene contents of crude oil emulsions. The performances of ten chemical demulsifiers on destabilization of five crude oil emulsions of different asphaltene contents from different oil wells were studied. Results showed that the viscosity of the emulsions increases as the water content increased. One of the emulsions, Seplat Oben flow station emulsion, however behaved differently. The difference in behavior was accounted for by phase inversion of the emulsion from water-in-oil emulsion to oil-in-water emulsion due to its high water content. There was no significant influence of whole asphaltene content on demulsifier performance for the studied emulsions. It is therefore, recommended that the resin content of the crude and the solvency of the asphaltene in each emulsion be studied and correlated with emulsion stability so as to shed more understanding on the influence of asphaltene on demulsifiers performances and stability of crude oil emulsions. The findings suggest that demulsifier screening be performed on each well and the best performing demulsifier adopted for that well.