Abstract: This study investigated the effectiveness of the use of cow urine in the control of microbially induced corrosion in oil transmission pipelines. Hydrogenase test was carried out and used to detect the presence of micro-organisms that cause corrosion in the pipeline samples. The cow urine biocide was subjected to urinalysis test after which it was characterized. The biocides were then subjected to experimentation to ascertain how effective they can be in reducing microbial corrosion using coupons filled with bacterial cultured agar. Weight loss and the corrosion rate of the samples were determined at weekly intervals throughout the duration of the experiment. The results show that cow urine coupon witnessed the least weight loss amounting to 0.9 g throughout the duration of the experiment. The other biocides formaldehyde, polyamine, diamine and biguanide have weight loss of 6.6, 5.8, 6.81 and 1.2 g, respectively. The 5 polynomial equations were formulated for weight loss for each of the biocides. R² goodness-of-fit statistical technique was employed in the analysis for future predictions from the model and the R² values obtained in each case is close to 1 which indicates a good measure that future outcomes are very likely to be predicted well by the obtained polynomial equations. Results also show that cow urine has an average corrosion rate of 0.0686 mm year^-1, as against the formaldehyde, polyamine, diamine and biguanide which have on the average 1.687, 1.7817, 1.8004 and 0.2225 mm year^-1 corrosion rates, respectively. Hence, it can be concluded that cow urine is the most potent biocide followed by biguanide out of the rest treated in this study for microbial corrosion.