Authors : D. Oshanova, D. Bayanbek, S. Anuarbekova, B. Atabaeva, A. Abdraimova, V. Remele and G. Ospankulova
Abstract: This research studied the ability to produce proteolytic enzymes in 27 fungal strains isolated from different crops. Proteolysis of casein and gelatin is studied. Proteolytic activity based on the modified method of Anson was evaluated. It is shown that fungi exhibiting high proteolytic activity during casein liquefaction may also show high activity and decomposition of gelatin. In spectrophotometric study showed the most active strains of Penicillium bilaiae 3-4,1 μM/mL, Penicillium chrysogenum 34/307-3,60 μM/mL, Aspergillus fumigatus 63/360-7,1 μM/mL. It also showed high numbers of casein liquefaction. Genotyping of six active cultures was made by direct method for determining the nucleotide sequence of ITS region (transcribed intergenic region) followed by determination of nucleotide identity with the sequences deposited in the international data base of Gene Bank. Identification was carried out by combination with phenotypic traits. Fungal culture in an amount of 27 will enrich the collection of the Institute.
D. Oshanova, D. Bayanbek, S. Anuarbekova, B. Atabaeva, A. Abdraimova, V. Remele and G. Ospankulova, 2017. Obtaining and Genotyping of Fungal Producers of Proteolytic Enzymes. Journal of Engineering and Applied Sciences, 12: 1920-1930.