Authors : Norain Mohd Nor, Shamsiah Abdullah, Abdul Rahim Harun, Nor`Aishah Hassan and Sopiah Mat Desa
Abstract: Rice is an important agricultural crop particularly in Malaysia. The rice production was affected by many factors including rice blast disease. In attempt to overcome the rice blast disease, genetic analysis study on new crosses of cultivar from Pongsu Seribu 2 and MR264 was used. The objective of this study is to identify segregation pattern of F2 and F3 population resistance to M. oryzae at genotypic and phenotypic level with associated resistance genes linked with SSR markers. SSR markers were used to screen segregating pattern in genotypic level while phenotypic screening was done in greenhouse by observing the disease development to the rice plant. Chi-square results shows a good fit of 1:2:1 for a single gene model which proved the hypothesis of a single dominant gene and 3:1 for the segregation of resistance and susceptibility for M. oryzae but did not segregate with epistasis and two-gene model. SSR markers of RM101, RM206, RM413 and RM495 were significantly associated with blast resistance to M. oryzae in rice. These markers were highly accurate for resistant plant with resistance effect about 12% for phenotypic variation. This study also shows that there was a strong interrelationship between the selected phenotypic and the four markers in this study. Therefore, these studies can be utilized for future references to identify these resistant and susceptible varieties based on polymorphism. This research offered very useful information for local rice breeders in developing blast disease resistant rice cultivar.
Norain Mohd Nor, Shamsiah Abdullah, Abdul Rahim Harun, Nor`Aishah Hassan and Sopiah Mat Desa, 2017. Inheritance Study of Blast Resistance with Pathotype P7.2 on Crosses of Pongsu Seribu 2 and MR264. Journal of Engineering and Applied Sciences, 12: 2633-2639.