Abstract: Organizations use accounting information system for data processing of financial and non-financial into financial information which is required by the user for decision making. The accounting information system is influenced by various factors in the organizational environment. Organizational culture is as one of the organization’s internal environmental factors which influence the implementation of accounting information systems. This study is aimed to examine the influence of organizational culture on the use of accounting information systems and the tests are conducted at 33 hospitals in Riau province which are selected by using simple random sampling techniques and analysis tools of simple regression. The study concluded that organizational culture significantly influences accounting information system. The results of this study can be used to solve the problem of the use of accounting information systems through the improvement of organizational culture.