Authors : Nurul Izzati Osman, Norrizah Jaafar Sidik and Asmah Awal
Abstract: The present study was conducted to initiate and establish a homogeneous cell suspension culture system in Barringtonia racemosa from endosperm-derived friable calli which are useful for plant secondary metabolites study. Initiation and establishment of cell suspension cultures study were carried out by using different treatments of liquid media involving different concentrations and combinations of 2, 4-D (0.5 and 1.0 mg/L) and kinetin (0.75, 1.5 and 2.0 mg/L) plant hormones in MS (Murashige and Skoog’s) medium. The difference in the treatments was formulated based on the reference treatments which produced the most optimum friable calli suitable for cell suspension cultures initiation. The treatment consisted of 1.0 mg/L 2, 4-D and 1.5 mg/L kinetin (treatment A) was found to be the most optimum treatment by considering timely mean dry cell biomass changes at every 5 days, interval with its maximum record of 16.32±0.72 g/L. The distinctive phases of cell growth involving lag, exponential and declining phases had been identified. However, no lag phase were identified from the optimum treatment while other two treatments (1.0 mg/L 2,4-D + 2.0 mg/L kinetin (treatment B) and 0.5 mg/L 2,4-D+0.75 mg/L kinetin (treatment C)) underwent lag phase during first 10 days in culture. These were followed by exponential phases which were continued and achieved the peak of highest dry cell biomass records on day 50 (treatment A and C) and 55 (treatment B). Afterwards, all treatments had shown a declining progress in mean dry cell biomass records. The findings pertaining to cell growth phases are providing a useful information on plant cellular and molecular processes studies in this species and would further be utilised in research related to plant secondary metabolites production.
Nurul Izzati Osman, Norrizah Jaafar Sidik and Asmah Awal, 2018. Establishment of Cell Suspension Cultures for Plant Secondary Metabolites Study in Barringtonia racemosa L.. Journal of Engineering and Applied Sciences, 13: 3119-3124.