Abstract: To construct the micro porous anode, stainless steel fiber felts are coated with the polyaniline. In this study, two methods are used to synthesis the nano-polyaniline. They are electrochemical oxidative and chemical oxidative polymerization methods. This three Dimensional (3D) anodes give more reaction because of its increased surface area. More the surface area more the reaction takes place. It’s also decomposable because it gives more area for bacterial colonization. The weight is reduced comparatively, so, it’s easy to transportation purposes. Polyaniline modified anodes give higher power output and decrease internal resistance. EIS with the polarization curves clearly explains this output. The result also shows polyaniline combining with the 3D macroporous metallic scaffold is a method for microbial fuel cell anode fabrication.