Authors : Azman Abdullah, Nadia Salem Alrawaiq and Ahmed Elbadri
Abstract: Heme Oxygenase-1 (HO-1) is an enzyme that possess anti-oxidant, anti-inflammatory and cytoprotective functions. Induction of HO-1 occurs as an adaptive and beneficial response to various injurious stimuli such as oxidative stress. This study aimed at monitoring the effects of administration of equal doses (50 mg/kg) of sulforaphane, curcumin and quercetin for 14 days on the levels of liver HO-1 gene and protein expression in mice. The 24 ICR male white mice (25-30 g) were divided into 4 groups: the control (vehicle treated) group (n = 6) the sulforaphane treated group (n = 6), the quercetin treated group (n = 6) and the curcumin treated group (n = 6). The chemicals were dissolved in a vehicle (DMSO, Tween-20 and normal saline in the ratio of 0.05: 0.1: 0.85) and administered intraperitoneally at a dose of 50 mg/kg for 14 days. Only the vehicle was administered to the control group. At day 15, animals were sacrificed and their livers isolated. Total RNA was extracted, reverse transcribed and subjected to quantitative real-time PCR to detect HO-1 gene expression. Agarose gel electrophoresis was also performed to verify amplification specificity. HO-1 protein expression was determined by Western blotting. HO-1 gene expression showed significant increase of 4.6, 3.3 and 3.0-fold and HO-1 protein expression showed significant increase of 2.3, 1.8 and 1.7-fold following treatment with sulforaphane, curcumin and quercetin respectively. Sulforaphane had the highest impact on the induction of HO-1 expression in mice liver, followed by curcumin and quercetin.
Azman Abdullah, Nadia Salem Alrawaiq and Ahmed Elbadri, 2018. The Effects of Equal Doses of Sulforaphane, Curcumin and Quercetin on Heme Oxygenase-1 Gene and Protein Expression in Mice Liver. Journal of Engineering and Applied Sciences, 13: 8885-8889.