Authors : Mi-Soon Park, Mi-Hwa Lee and Byung-Soo Jang
Abstract: To investigate the structure of biofilm and the micro-structural characteristics of anaerobic Gram-positive Actinomyces by vaginal examination and microscopic analysis of the secretions from infected patients.The presence or absence of sulfur granules produced by these bacteria were analyzed using energy dispersive X-ray spectroscopy (INCA., Oxford Ins., UK) and clinical specimens from the patients infected with Actinomyces were collected for analysis via. Scanning Electron Microscopy (SEM). From high resolution Scanning Electron Microscopy (SEM), the surface of hypha-formed biofilmwas covered with a substance secreted by the bacteria and hexagonal sulfur granules were scattered aroundthe mycelium. The formation of the porous biofilm is believed to create the best conditions for bacterial growth and proliferation in the anaerobic state. Granules of 100-150 nm in size were observed and the components were measured by Energy Dispersive X-ray spectroscopy (EDX). Vaginal smear specimens of squamous intraepithelial cells were observed through optical, electronic and transmission electron microscopy and the bacteria were shown to produce an electrolyte salt. The most common of the bacteria is parasitic in the host and creates major growth of a biofilm that maintains a persistent infection. Ecosystems of the vagina are affected by a number of different types of microorganisms, pH and concentration of sugar. These ecosystems can also be affected by hormonal changes,medications, douches and frequency of sexual intercourse. Thus, it is considered that one should maintain personal hygiene such that the vaginal pH stays slightly acidic and one should use vaginal cleaning agents with special care, since, frequent use is considered to cause hypersensitivity reactions, total destruction of normal bacteria and a lowering of the vaginal pH.
Mi-Soon Park, Mi-Hwa Lee and Byung-Soo Jang, 2018. A Study on the Ultra Structure of Actinomycosis in Women with IUDs. Journal of Engineering and Applied Sciences, 13: 9320-9326.