Abstract: The research aims to identify the types of cultures applied in the Gulf Commercial Bank as well as the effect of the organizational culture of all types bureaucratic culture, clan culture, entrepreneurship culture and market culture on the quality of service with its subsidiary dimensions reliability, responsiveness, Assurance, empathy, tangibility. In order to achieve the objective of the research, a questionnaire was prepared for each variable. The number of paragraphs for the first variable was 12 and the questionnaire was distributed on the employees at the bank. The number of paragraphs for the second variable was 15 and the questionnaire was distributed on the bank customers to determine their opinions about the quality of service provided by the bank. The number of the distributed formsis (30). The statistical data were processed and the results were analyzed in light of the outputs of the statistical program SPSS. The most important findings of the research were the existence of a strong relationship between the variables and the hypothesis proved correct. The Gulf Commercial Bank applies the market culture in the first place. The entrepreneurship culture and the tribe culture attained a close proportion and does not differ significantly from the market culture while the bureaucratic culture received the lowest percentage. This indicates an increase in awareness in the application of modern cultures.