Authors : A. M. Sahilah and Radu Son
Abstract: A total of 32 isolates of Salmonella muenchen isolated from from poultry were examined for antibiotic resistance, plamid profiling and random amplified polymorphic DNA (RAPD-PCR). The antibiotic-resistant of 32 S. muenchen isolates were classified into 4 groups (Group I-IV) on the basis of their susceptibility to penicillin G, streptomycin, tetracycline, kanamycin and carbenicillin. All isolates showed resistance towards penicillin G (100%) and tetracycline (100%) and 87.5% toward streptomycin. None is resistant to cephalothin and nalidixic acid. All isolates carried atleast one plasmid ranging in sizes from < 1.3 to 47.0 megadalton (MDa) that enabled the S. meunchen to be grouped into twenty seven plasmid profiles. The RAPD-PCR results showed that the collection of isolates were genetically very heterogenous. The combination of three primers used enabled us to differentiate the 32 S. muenchen isolates into 32 differences genome types. Our results demonstrate that RAPD-PCR fingerprinting method is more sensitive than antibiotic resistance patterns and plasmid profiling with respect to the individualisation of the isolates used in this study.
Sahilah, A. M. and Son, Radu , 2003. Typing of Salmonella muenchen Isolated from Poultry by Antibiotic Resistance, Plasmid Profiling and Random Amplified Polymorphic DNA-PCR . Journal of Food Technology, 1: 54-62.