Authors : A. Anene, U.H. Ukpabi, J. Ibewuike and V.N. Jumbo
Abstract: The aims of this study, include to assess the haematological responses of Clarias gariepinus fed diets containing Boiled Mucuna Seed Meal (BMSM). The seed meal was incorporated in diets at 0, 25, 50, 75 and 100% of experimental diets. All diets were iso-nitrogenous (39.66-40.29% crude protein). Fingerlings of Clarias gariepinus with initial mean weight of 10.15±1.54 g were fed ad lib. on allotted diets twice per day at 08.00 and 18.00 h for 60 days. The experiment was a complete randomized design. Fishes fed 25% BMSM had highest white blood cell count values of 15.93±0.23x106 mL-1 while the lowest value of 6.00±0.28x106 mL-1 was recorded in fishes that were fed with the diet containing 100% BMSM. Haemoglobin (Hb) levels in experimental fish fed experimental diets containing 25 and 50% BMSM was 6.53±0.38 and 6.40±0.67%, respectively and significantly decreased to 4.25±0.05 in fishes fed diets with 100% BMSM. The results for the packed cell volume were 36.3±0.39% for fish fed on control diet, 26.23±0.78% for T2, 26.55±0.71% for T3, 23.88±0.41% for T4 and 17.05±0.09% for T5. Values obtained for Mean Cell Haemoglobin (MCH) were 17.71±1.09% for T1, 19.32±0.97% for T2, 20.78±1.72% for T3, 18.62±1.08% for T4 and 23.88±0.36% for T5. Protein concentrations in blood of experimental fish fed the control diet T1 was 4.03±0.87 and 3.53±0.54 mg dL-1 in T2. In T3 and T4 total protein concentrations were 2.90±0.04 and 3 mg dL-1 and 2.58±0.35, respectively. Urea results were 20.13±2.36 mg dL-1 for fishes fed diet T1, 19.43±2.17 mg dL-1, for those fed diet T2, 20.50±1.16 mg dL-1 for fishes fed diet T3 and 21.35±2.07 mg dL-1 for those diet T4 and 21.90±1.67 mg dL-1 for T5. Alanine transaminase values of the fish were 8.33±1.23, 8.20±1.44, 7.3±1.65, 8.05±0.88 and 10.15±1.13 U I-1 in fishes fed diets T1-5, respectively. Aspertate transaminase concentrations in the blood of experimental fish fed the following experimental diets T1-T5, respectively. Blood serum of C. gariepinus fed on the following experimental diets T1-T5 contained the following concentrations of alkaline phosphate 25.43±2.33, 26.20±1.58, 25.83±1.76, 24.55±1.87 and 24.30±1.99, respectively.
A. Anene, U.H. Ukpabi, J. Ibewuike and V.N. Jumbo, 2014. Some Haematological Responses of Fingerlings of Clarias gariepinus Fed Different Dietary Levels of Boiled Mucuna cochichinensis Seed Meal (BMSM). Journal of Aquaculture Feed Science and Nutrition, 6: 51-58.