Abstract: Effects of seed material, amount and type of organic soil amendment on root-gall nematode disease of pineapple were assessed. The study was conducted in a 3�3�3 factorial experiment in randomized complete block design replicated 4 times. Planted pineapple crowns, slips and suckers were treated with 0.00, 1.50 and 3.00 tons ha ¹ of poultry manure, sawdust and pigdung. Results showed that plants propagated from pineapple crowns were most susceptible to the disease at 0.00 tons ha ¹ of organic soil amendment. The disease however decreased in all the seed materials as rates of the organic soil amendments increased. At 3.00 tons ha ¹ of poultry manure application, no root-gall damage occurred in any of the pineapple plants. Highest fresh fruit weight obtained on crown propagated plants occurred at this 3.00 tons ha ¹ of poultry manure application.