Abstract: In aquaculture industry, spermatophore transfer technique such as artificial insemination in particular with reference to banana shrimp P. merguiensis is challenging. The aim of this study is to examine a novel technique for artificial insemination in P. merguiensis using SHDAI (Shrimp Holder Device for Artificial Insemination). In order to transfer the spermatophore properly into thelycum, an appropriate shrimp holder with continuous aeration system has been developed. During the process of manual spermatophore transfer (artificial insemination) also, a protective device to keep the female P. merguiensis shrimps alive and under minimum stress is necessary. The artificial insemination process carried out using SHDAI showed no signs of stress and/or mortality of the broodstock. During the experiments, female shrimps were 100% alive and active. Altogether, 78 female shrimps were tested of which 63 successfully accepted the spermatophore by using SHDAI. The accepted spermatophore percentage was significantly higher and achieved as 80.76%. Accepted spermatophore mass in the frozen, control and mean of both were recorded as 48.36±10.45, 45.0±15.28 and 46.68±2.38% which indicated no significant differences (p<0.05) However, frozen sperm at -196°C LN up to 90 days was 64.1±4.3% which indicated no significant differences between the SHDAI and control (p<0.05). Whereas frozen sperm at 196°C up to 90 days in LN was 62.5±2.9% there was no significant difference between the SHDAI and control (p<0.05). In the present study, overall quality of sperm in terms of the fertilization rate and hatching rate were almost similar between inseminated (using SHDAI) and control (natural mating) broodstock.
A.J. Memon, N. Hidayati, A.D. Talpur, M.I. Khan, M.O. Fariddudin, J. Safiah, M. Ikhwanuddin and A.B. Abol-Munafi, 2012. Development of Shrimp Holder Device for Artificial Insemination of Banana Shrimp Penaeus merguiensis (De Man, 1888). Research Journal of Applied Sciences, 7: 10-16.