Authors : Zohreh Pouretedal and Azamosadat Meshkani
Abstract: Two different clean-up methods for determination of Deoxynivalenol (DON) in cereals by liquid chromatography with DAD detection were compared. The efficiencies of two clean-up principles for rice extracts were compared: solid-phase and immunochemical extraction. There are advantages and disadvantages with both methods. In aspect of recovery, Immunoaffinity Columns (IAC) seemed to be more efficient but Solid Phase Extraction (SPE) column are more precise. Although both methods meet the requirements for reliability, for unknown matrices SPE method is somehow unreliable and has false positives. In terms of price SPE is less expensive because of self-made columns but IAC clean-up method is a specific method.
Zohreh Pouretedal and Azamosadat Meshkani , 2008. Comparing Determination of Deoxynivalenol in Cereals by HPLC Using Two Different Clean-Up Methods. Research Journal of Biological Sciences, 3: 968-972.