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Research Journal of Biological Sciences
Year: 2009 | Volume: 4 | Issue: 9 | Page No.: 1056-1060
Antibacterial Effect of Synthesized Silver/Montmorillonite Nanocomposites by UV-Irradiation Method
Mansor Bin Ahmad , Majid Darroudi , Kamyar Shameli , Abdul Halim Abdullah , Nor Azowa Ibrahim , Azizah Abdul Hamid and Mohsen Zargar
Abstract: Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by UV-irradiation method. AgNO3 and Montmorillonite (MMT) were used as a silver precursor and solid support, respectively. The properties of Ag/MMT nanocomposites were studied as a function of UV-irradiation time. UV-irradiation disintegrated the Ag NPs into smaller size until a relatively stable size and size distribution were achieved. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing UV-irradiation time. The synthesized Ag/MMT nanocomposites under longer UV irradiation time are very stable over a long time in aqueous solution without any sign of agglomeration or precipitants. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and Gram-positive bacteria (Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA)) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. All of synthesized Ag-NPs were found to have high antibacterial activity. These results showed that Ag-NPs can be useful in different biomedical research and applications such as diagnostic and surgical devices, contrast agents, drug delivery vehicles and physical therapy applications.
How to cite this article:
Mansor Bin Ahmad, Majid Darroudi, Kamyar Shameli, Abdul Halim Abdullah, Nor Azowa Ibrahim, Azizah Abdul Hamid and Mohsen Zargar, 2009. Antibacterial Effect of Synthesized Silver/Montmorillonite Nanocomposites by UV-Irradiation Method. Research Journal of Biological Sciences, 4: 1056-1060.