Abstract: To compare mothers and nurses viewpoint about stressors for parents with premature newborn in NICU. Identification of the sources of NICU-related parental stress and quantification of its degree is essential to determine effective strategies to promote optimal parenting in this high risk setting. A comparative descriptive design was used. Data were collected by PSS questionnaire. The study population consist of all mothers with hospitalized premature newborn in NICU (n = 300) and nurses (n = 32) in three teaching hospital in Tabriz city (Iran). Validity of scale was determined by content validity with cooperation of ten faculty members of Nursing-Midwifery School of Tabriz and validity of translation assessed by one English language expert. Reliability of scale was evaluated with Cronbach’s alpha (mothers = 0.87 and nurses = 0.94). The most stressor from viewpoint of mothers was relationship with the baby and parental role and from viewpoint of nurses were baby looks, behaves and treatments and relationship with the baby and parental role. Comparison viewpoint of mothers and nurses showed significant difference between them. Present study showed that there is significant difference between nurses and mothers viewpoints about parents of premature babies hospitalized in NICU. So, nurses closely assessment of parent’s stressors especially, mothers of premature babies hospitalized in NICU is necessary for delivering family based care and making them satisfied with nursing services.