Research Journal of Biological Sciences

Year: 2009
Volume: 4
Issue: 8
Page No. 933 - 936

Investigation of Leptin’s Receptor Gene Polymorphism, by Using PCR-RFLP Technique in Native Poultry Population of Khouzestan Province

Authors : S. Mokhtarzadeh, J. Fayazi, M. Beigi Nasiri and K.H. Alamisaeed

Abstract: Leptin, the product of the obese (ob) gene, is a 16-kDa hormone that has been shown to play an important role in the regulation of food intake, energy expenditure and hypothalamus endocrine function in response to nutritional changes. Chicken leptin receptor is expressed in the hypothalamus, but also in other tissues such as pancreas, where leptin inhibits insulin secretion and thus, may have a key role in regulating nutrient utilization in this species. However, further studies have shown that leptin receptors are also expressed in many other tissues and have suggested that leptin is involved in more diverse biological functions than previously thought. The ability of insulin and leptin to regulate chicken leptin receptor gene expression suggests a direct role of leptin in the control of hepatic metabolism. In order to study of Leptin’s Receptor gene (LEPR) polymorphism, by using PCR-RFLP technique in native poultry population of Khouzestan province, a research was planed and done in molecular lab of Ramin agricultural and natural resources. In this research, a fragment of extracted DNA from native poultry blood's samples was amplified with PCR technique. This 2900 bp fragment consists of number 9-11 exon's of LEPR. This fragment was digested with HaeIII restricted enzyme. The pictures that were tacked from agarose gel showed 2 allele (A and B) for this fragment. The allele frequencies were 31.19 for A and 61.81 for B. The genotype frequencies were 18.81, 24.75 and 54.44 for AA, AB and BB genotypes, respectively. The studied population was in Hardy-Weinberg disequilibrium.

How to cite this article:

S. Mokhtarzadeh, J. Fayazi, M. Beigi Nasiri and K.H. Alamisaeed, 2009. Investigation of Leptin’s Receptor Gene Polymorphism, by Using PCR-RFLP Technique in Native Poultry Population of Khouzestan Province. Research Journal of Biological Sciences, 4: 933-936.

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