Authors : Seifollah Bahramikia, Razieh Yazdanparast, Alireza Rezazadeh and Samaneh Bayati
Abstract: Oxidative damage to proteins and lipids leads to severe failure of their biological functions with subsequent final effects on cells functions in various tissues especially liver and brain. In this study, researchers investigated the protective effects of two salen free radical scavengers namely EUK-8 and -134 and also a standard antioxidant (catechin) in two different models of oxidative stress. In the first study, researchers used Fe2+/Ascorbate Model as a well-validated system for production of ROS in rat liver homogenates and the protective effects of EUK-8, EUK-134 and catechin against this system were evaluated. In the second study, the protective capabilities of these compounds against the free-radical damaging effects of hydrogen peroxide (H2O2) on SK-N-MC cell line were evaluated in term of attenuation of intracellular lipofuscin level. Results of the first study indicated that the simultaneous addition of Fe2+/ascorbate and EUK-8 and/or EUK-134 at different concentrations (5, 10, 25 and 50 μM) to the liver homogenate significantly decreased the extent of PCO, LPO and ROS while the level of PB-SH increased relative to the control group. Results of the second study also showed that pretreatment of the cells with EUK-8 and -134 (25 μM) followed by exposure to H2O2 restored the viabilities of cells relative to the H2O2-treated cells. In addition, each of the compounds significantly and time-dependently reduced the intracellular level of lipofuscin pigments among the H2O2-treated cells.
Seifollah Bahramikia, Razieh Yazdanparast, Alireza Rezazadeh and Samaneh Bayati, 2011. Salen-Manganase Complexes, EUK-8 and EUK-134, Protect Against Protein and Lipid Oxidative in Neuroblastoma Cells and Rat Liver Homogenates in vitro. Research Journal of Biological Sciences, 6: 670-677.