Authors : Sadegh Abedi, Fatemeh Ehtesham Far, Mohd. Khairi Hussain, Zuraini Ahmad and Abdul Manan Mat Jais
Abstract: Inflammation is a non-specific reaction of body tissues against harmful stimulus, injury or infection to protect the body and promote the recovery process. Myeloperoxidase a pro-inflammatory enzyme released by activated neutrophils and macrophages is frequently used as a marker of polymorphonuclears accumulation in different tissue samples. Haruan (Channa striatus) is a tropical, snakehead, freshwater, air breathing and carnivorous fish belongs to family channadae which is indigenous to Malaysia. The current study was undertaken to identify the properties of a new haruan based cream as a natural product on myeloperoxidase enzyme as a marker of polymorphonuclears accumulation. In the present study, anti-inflammatory activity of haruan (Channa striatus) based cream (at doses 1, 5 and 10%) on the ear thickness and myeloperoxidase activity (as two acute inflammation indexes) were studied using croton oil induced ear edema method and compared with effects of hydrocortisone 1% cream as reference drug. Results showed that all percentages of haruan based cream in a same potent with reference treatment significantly inhibited edema at 4 and 24 h after croton oil application (p<0.05). Besides, myeloperoxidase assay results shown that topical application of these three different dosages of haruan based cream also block migration of polymorphonuclear leukocytes to the dermis as effective as hydrocortisone cream 1% and in a dose dependent manner (p<0.05). Evidences obtained from the present study, on the one side, support the previous results that had shown the anti-inflammatory effects of haruan extracts in other animal models and on the other side, introduce a new topical biomedical product with a potency as great as hydrocortisone in inhibition of inflammation. It is suggested that topical anti-inflammatory effects of haruan based cream is contributed to its bioactive substances content such as linoleic acid, stearic acid, oleic acid and N-arachidonylplycine.
Sadegh Abedi, Fatemeh Ehtesham Far, Mohd. Khairi Hussain, Zuraini Ahmad and Abdul Manan Mat Jais, 2012. Effects of Haruan (Channa striatus) Based Cream on Acute Inflammation in Croton Oil Induced Mice Ear Edema Model. Research Journal of Biological Sciences, 7: 181-187.