Authors : Fereshteh Golfakhrabadi, Seyed Nasser Ostad, Mitra Hafizi, Mohammad Reza Shams Ardekani, Soodabeh Saeidnia, Tahmineh Akbarzadeh and Mahnaz Khanavi
Abstract: Ferulago carduchorum Boiss and Hausskn (Apiaceae) is an endemic plant in West of Iran and it are used in dairy and oil ghee as a natural preservative. The aerial parts of F. carduchorum were extracted by percolation method with MeOH/H2O (80/20) and the fractions were provided respectively with hexane, ethyl acetate, methanol, methanol/H2O (50/50). The fractions have been investigated their phytochemical screening and the cytotoxic activity against the colon carcinoma (HT29), the breast ducal carcinoma (T47D), the hepatocellular carcinoma (HepG2) and Swiss mouse embryo fibroblast (NIH 3T3) cell lines by MTT (3-(4, 5-di methyl thiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Phytochemical screening revealed the presence of phytosteroids, flavonoids, coumarins and saponins. The cytotoxicity of ethyl acetate and hexane fractions showed more efficacy than the other fractions on T47D and HepG2 cell lines (IC50<100 μg mL-1) could be attributed to their content of coumarins and phytosteroids. The hexane fraction was selected for phytochemical study and suberosin (a coumarin) isolated from hexane fraction as an active compound. The Selective Index (SI) value of ethyl acetate fraction was lower in HepG2, T47D and HT29 cell lines than hexane fraction that indicated the selective effect of ethyl acetate fraction on this cell lines.
Fereshteh Golfakhrabadi, Seyed Nasser Ostad, Mitra Hafizi, Mohammad Reza Shams Ardekani, Soodabeh Saeidnia, Tahmineh Akbarzadeh and Mahnaz Khanavi, 2013. Phytochemical Analysis and Cytotoxic Activity of Ferulago carduchorum. Research Journal of Biological Sciences, 8: 138-142.