Authors : Nining Hong, Tian Jiesheng and Li Jilun
Abstract: To improve the efficiency of magnetosome extraction, a lysozyme expressing strain of Magnetospirillum gryphiswaldense MSR-1, named MLE was constructed. Compared to wild type, MLE showed no significant differences in cell growth and magnetosome formation. Colorimetric monitoring and transmission electron microscopy confirmed that MLE cells could be disrupted more effectively following freeze thaw treatment. Importantly, extraction of magnetosomes from the engineered strain could be easily performed on a large scale.
Nining Hong, Tian Jiesheng and Li Jilun, 2017. Heteroexpression of Bacteriophage T7 Lysozyme in Magnetospirillum gryphiswaldense Facilitates Large-Scale Extraction of Magnetosomes. Research Journal of Biological Sciences, 12: 6-12.