Authors : V. Tessitore , M.L. Uzzo , G. Bonaventura , F. Carini , G. Milio and G.F. Spatola
Abstract: The pathophysiologic mechanism related to pathogenesis of cutaneous trophic disorders in Chronic Venous Insufficiency (CVI) find different explanations. The increase in the number of capillaries and the subsequently stasis, the fibrin cuffs deposited around the capillaries and leucocytes migration associated at inflammatory phenomena in fact try to explain the etiology of venous ulceration. In this study, we have evaluate the immunohistochemical presence of iNOS and eNOS in cutaneous bord of venous ulcers to evaluate the role of Nitric Oxide (NO) in pathophysiology of these trophic disorders. In addition, using the image analisys method, we have evaluate the increase of capillary numbers respect to normal skin. We have studied immunohistochemical distribution of iNOS and eNOS in human normal skin and in peripheral bord of venous ulcers in 20 subject affected by CVI. The specimens have been taken during a surgical treatment. All of these are fixed in Bouin�s mixture and processed with eNOS (Transduction N30020) and iNOS (Transduction N32030) antibodies. After the immunostaining procedures all specimens are studied with Nikon microscope and Lucia system of image analisys. Moderate eNOS immunoreactivity are expressed in the same way both in the endothelium of a dermic capillaries and in the spinous epithelium of the normal skin such as in the pathological specimens. iNOS immunopositivity is more expressed in the spinous epithelium and in the capillaries endothelium of a bord of venous ulcer than in a normal skin. At the image analisys the capillary numbers in the venous ulcers is remarkably higher respect the normal skin. These data provide a morphological basis to explain a possible pathogenesis in the cutaneous trophic disorders in CVI. In fact the strong iNOS immunoreactivity, expressed in this inflammatory phenomenon, may be implicated in microcirculatory stasis.
V. Tessitore , M.L. Uzzo , G. Bonaventura , F. Carini , G. Milio and G.F. Spatola , 2007. NO and Chronic Venous Insufficiency (CVI): ImmunohistochemicalEvaluation of iNOS and eNOS Isoforms in the Venous Ulcers. Clinical and Therapeutical Possible Implications . Research Journal of Medical Sciences, 1: 230-233.