Authors : Elena T. Zhilyakova, Marina Y. Novikova, Oleg O. Novikov, Dmitriy I. Pisarev and Alexandra V. Baskakova
Abstract: An original method for analyzing substances, ciprofloxacin, acyclovir and cyanocobalamin with which it will be possible to carry out a study of ophthalmic dosage forms containing these components. This is used converts-phase chromatography option. As eluting system to study components chosen mixture of 1% aqueous formic acid-ethyl alcohol in a ratio of 10:90. Since, all components are well or moderately soluble in alcohol, using a mobile phase of 90% of the solvent yielded the optimum retention time of the compounds. Another important advantage of this system is that carried out the replacement of expensive and toxic acetonitrile is traditionally used in the world practice in the method converts phase HPLC on non-toxic and available ethyl alcohol. The quality of the results of chromatography is not treason. To register the results used diode-array detector, registering the electronic absorption spectra which is an objective in the case of the analysis of these compounds because they all have characteristic absorption bands in the UV and visible regions of the spectrum. Analysis time was no >3 min which is a characteristic of the chromatographic process to be very rapid.
Elena T. Zhilyakova, Marina Y. Novikova, Oleg O. Novikov, Dmitriy I. Pisarev and Alexandra V. Baskakova, 2015. The Optimization of the Method of Analysis of Ciprofloxacin, Aciclovir and Cyanocobalamin to Ensure Quality Control in the Developed Domestic Ophthalmic Dosage Forms. Research Journal of Medical Sciences, 9: 208-213.