Authors : Siti Fathiah Masre, George W. Yip, K.N.S. Sirajudeen and Farid Che Ghazali
Abstract: Massive nutritional and medicinal values of sea cucumbers have long been reported in the past that may be due to sulfated Glycosaminoglycan (GAG). Thus, the effects of total sulfated GAG from local Stichopus vastus coelomic fluid were analyzed for its potential in full-thickness excisional wound healing. The 20 μL of 1 μg mL-1 total sulfated GAG was applied topically to 6 mm diameter of full thickness wound from day 0-12 on the dorsal part of female sprague-dawley rats (n = 10). Whilst control group was treated with 20 μL of normal saline. Wound healing activities were assessed through wound contraction rate (%) and semi-quantitative measurement on the histology analysis of epithelial migration, inflammatory responses, fibroblasts proliferation, new vessels formation and collagen organization. Results showed significantly improve wound contraction rate on each observation day in the treatment group compared to the control group. Total sulfated GAG from S. vastus coelomic fluid showed significantly acceleration on wound healing process in epithelial migration, fibroblasts proliferation, new vessels formation and collagen organization. Data strongly documented the wound healing effects of sulfated GAG from the local sea cucumber S. vastus coelomic fluid.
Siti Fathiah Masre, George W. Yip, K.N.S. Sirajudeen and Farid Che Ghazali, 2015. Wound Healing Potential of Total Sulfated Glycosaminoglycan (GAG) from Malaysian Sea Cucumber, Stichopus vastus Coelomic Fluid. Research Journal of Medical Sciences, 9: 67-72.