Authors : Jing Huang , Zheng-Mao Zhu and Shu-Hong Zhao
Abstract: SDHD (Succinate Dehydrogenase D) is a maternally imprinted gene in human carotid body tissue. Mutations carried by the father of this gene may cause embryo growth retardation and tumor, therefore it is important to study the function of this gene. In this study, we detected the imprinting status of porcine SDHD in the 90 day prenatal muscle and placental tissues, analyzed the genotype frequencies of a non-synonymous BstF5I SNP in the 4th exon of this gene and detected the expression in prenatal muscle of three embryonic developmental stages (33d, 65d, 90d). The results showed that MobI PCR-RFLP from both DNA and cDNA from the same animal muscle and placental tissue are heterozygote, sequencing of both DNA and cDNA showed there is no preferential expression of any of the two alleles. Thus SDHD has no imprinting effect in porcine 90 day fetus muscle tissue and placenta tissue. The allele frequencies of BstF5I PCR-RFLP in different pig breeds revealed a much higher frequency of allele A in Chinese indigenous breeds Erhualian and Yushanhei pigs whereas allele G has higher frequency in Tongcheng, Large White and Landrance pigs. The RT-PCR result of SDHD showed that this gene is expressed in prenatal muscles in all the three embryonic developmental stages in the porcine.
Jing Huang , Zheng-Mao Zhu and Shu-Hong Zhao , 2007. SSNP Detection, Imprinting and Expression Analysis in Prenatal Skeletal Muscle of the Porcine Succinate Dehydrogenase Complex, Subunit D (SDHD) Gene . Research Journal of Animal Sciences, 1: 102-106.