Authors : Andriy Lyashenko and Mikhael Bashchenko
Abstract: The aim of this research was to improve the indicators of motility, survival and fertilizing ability of spermatozoa by optimizing temperature factors and the duration of exposure at unfreezing straws. Straws by volume 0.25 mL were thawed at water bath temperatures at 65, 67 and 70°C for 6-7 sec and at 75°C for 4-6 sec. Impact of exposure time and temperature thawing in the water bath on motility and survival of spermatozoa were studied. Studies indicate that for the procedure of defrost water bath straws in 7 sec for temperature conditions of 65, 67 and 70°C, indicators of progressive motility and absolute survival rate were significantly higher than for the control group an average on 11.4% (p<0.01). Optimum exposure time (6-7 sec) and temperature range (65-70°C) defrosting semen doses were defined. Owing obtained the positive result, method of thawing was developed which increases the indicators of motility, survival and fertilizing capacity of bull semen.
Andriy Lyashenko and Mikhael Bashchenko, 2014. Effect of Different Thawing Procedures on the Quality and Fertility of the Bull Spermatozoa. Research Journal of Animal Sciences, 8: 18-23.