Authors : Jhy-Ming Li, Yen-Ming Horng, Yi-Ting Chen, Mu-Chiou Huang and Chean-Ping Wu
Abstract: The present study investigated the genetic variations in the Ryanodine Receptor 1 (RYR1) and Heart Fatty Acid-Binding Protein (H-FABP) genes, responsible for Porcine Stress Syndrome (PSS) observed during auction in pigs. A total of 655 pigs were sampled during either transportation or auction period. A SNaPshot assay, combining multiplex PCR with multiplex primer extension assay was used for targeted detection of all mutations in one reaction. The most frequent RYR1 genotype was the recessive nn, followed by the heterozygous Nn and the dominant NN. Investigation of the H locus of the 5' upstream region in H-FABP showed that the genotype frequencies of HH, Hh and hh were 0.82, 0.16 and 0.03, respectively. Furthermore, analysis of the D and A loci in the intron 2 region of H-FABP showed that the genotype frequencies of DD, Dd and dd were 0.03, 0.39 and 0.59 and those of AA, Aa and aa were 0.47, 0.51 and 0.02, respectively. Thus, a high incidence of PSS was associated with HH, dd, Dd, Aa and AA and a low rate with Hh, hh, DD and aa. In conclusion, a positive correlation was found between the PSS development and SNP genotypes of RYR1 but not H-FABP.
Jhy-Ming Li, Yen-Ming Horng, Yi-Ting Chen, Mu-Chiou Huang and Chean-Ping Wu, 2017. Analysis of Genetic Variation in RYR 1 and H-FABP in PSS Pigs using SNP Genotyping. Research Journal of Animal Sciences, 11: 12-17.