Abstract: The environmental problems have currently been important issues that need to be solved through involvements of all parts. Educational institutes and communities are considered to be important for mechanisms to solve the problems. The production of I-san (Northeastern) folk songs to promote the conservation of natural resources and environment is an approach used to promote awareness toward the natural resources and environmental conservation of students and people in general. The purposes of this research were: to produce I-san folk songs and a training handbook; study the results of knowledge transfer about the environment of students through training classified by genders and years of study and investigate the satisfaction of villagers toward the knowledge transfer about the environment to promote knowledge, attitude and awareness about natural resources and environmental conservation through Pong Lang musical performance of students. The sample used in this research was 30 Mathayomsuksa 4-6 students of Srikaewprachasan School under the jurisdiction of Secondary Educational Service Area Office 28, Loengnoktha district, Yasothon province and 329 villagers in communities close to location of the school. The instruments used for data collection were: 13 I-san folk songs; a training handbook; a test of knowledge about natural resources and environmental conservation with 40 items, the difficulty between 0.20 and 0.80, the discrimination between 0.27 and 0.62 and the reliability for whole research of 0.87, a test of attitude toward the conservation of natural resources and environment with 20 items; a test of awareness concerning natural resources and environmental conservation with 70; 5-rating scale items, the discrimination between 0.20 and 0.91 and the reliability for whole paper of 0.98 and a questionnaire about the satisfaction of villagers toward the use I-san folk songs through Pong Lang performance of students to promote knowledge, attitude and awareness about the natural resources and environmental conservation. The statistics used for data analysis were mean, percentage, standard deviation, paired t-test and F-test (two-way MANCOVA). The research results revealed that the appropriateness of 13 I-san folk songs for the promotion of natural resources and environmental conservation of the people in Loengnoktha district, Yasothon province, evaluated by the experts was at the highest level. They were all consistent with the 4 years provincial development plan (2010-2013) of Yasothon province. The levels of knowledge, attitude and awareness about natural resources and environmental conservation of the students both in general and classified by genders and years of study were higher than those of before the training with statistical significance at the level of 0.05. However, there was no relationship between genders and years of study with knowledge, attitude and awareness about natural resources and environmental conservation. The satisfaction of the villagers toward I-san folk songs through I-san Pong Lang musical performance to promote knowledge, attitude and awareness about natural resources and environmental conservation of the students was generally at the highest level.