Abstract: Present investigation was launched to detect oxidative stress in pregnant and non-pregnant Murrah buffaloes during extreme ambiences. Serum oxidase and peroxidase profiling was used as a tool to detect oxidative stress. Blood samples of apparently healthy adult female Murrah buffaloes were collected to harvest sera during extreme hot cold and moderate ambiences. Moderate mean value of serum oxidase was 50.32±1.21 UL-1 and of serum peroxidase was 49.51±1.11 mUL-1. Extreme hot and cold ambiences resulted into a significant (p≤0.05) rise in the activities of serum oxidase and peroxidase. Animals were classified as non pregnant milch, pregnant milch and pregnant dry and activities of both the enzymes were compared. It was observed that mean values of both the enzymes in non-pregnant milch, pregnant milch and pregnant dry differed significantly (p≤0.05) from each other, respectively in all the ambiences. Non pregnant milch animals showed lowest activities whereas pregnant dry animals exhibited highest activities of both the enzymes. Multipara animals showed significantly (p≤0.05) higher activities than primipara. Serum oxidase and peroxidase are considered as important markers to assess oxidative stress. Increased activities of both the enzymes reflected the presence of oxidative stress in the buffaloes. It was concluded that extreme ambiences resulted into oxidative stress affecting the animals of all the physiological states. In each ambience the magnitude of increase was greater in pregnant dry animals. Trend was similar in all the ambiences but impact was maximum during hot ambience. Multipara animals showed greater changes than primipara. It can be stated that multipara and pregnant dry animals require proper care and supplementation of immuno-nutrients in order to protect them from the ensuing risk of oxidative stress. Further serum oxidase and peroxidase activities can be used effectively in detection of oxidative stress.
A. Joshi, N. Kataria, S. Sharma, A. Sharma, S. Arora, Abhimanu , R. Maan, S. Asopa, N. Arya, S. Khan and A.K. Kataria, 2013. Detection of Oxidative Stress in Pregnant and Non-Pregnant Murrah Buffaloes During Extreme Ambiences. Veterinary Research, 6: 27-31.