Abstract: The Calpain3 (CAPN3) gene, located within the confidence interval of the Quantitative Trait Loci (QTL) affecting meat tenderness, being regarded as a candidate gene for meat tenderness in cattle. In this study, SNPs in exon 23-intron 23 region of the yak CAPN3 was investigated from 1032 yak with Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP). Three Single-Nucleotide Polymorphisms (SNPs), resulting in three novel alleles were detected in this region, respectively. These variations included one synonymous in exon 23 and two SNPs in intron 23. Allele C was the most common allele (63.18%) in all of yak. These SNPs are the first report in yak CAPN3 and also suggest further analysis is required to explore the relations between variation sites and meat tenderness.
Li Zhang, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li and Wei Yan, 2013. Single Nucleotide Polymorphisms of the Calpain 3 (CAPN3) Gene in Yak. Agricultural Journal, 8: 119-122.