Authors : Xiaobo Zheng, Lijuan Qing, Zhiwan Zheng, Jiao Li, Rong Xiao, Libin Wang, Yinzhu Yao, Boqing Wu, Huihao Xu, Maosen Xu, Jing Li, Qun Wu and Liang Ge
Abstract: Leydig cells were isolated from the testis of 3 week old Rongchang piglets. Its activity, purity and function were qualified before being cultured in vitro. Then, they were respectively treated with 0.125, 0.25, 0.5, 1 and 2 μmol L-1 c-jun ASODNs and the expression of c-jun mRNA were detected by FQ-PCR. To study the effect of c-jun on proliferation of elemental status leydig cells in piglets, the leydig cells had been treatedwith c-jun ASODNs and the growth inhibition ratio of leydig cells were detected by MTT method and cell cycle phase distribution were examined by flow cytometer analysis. The FQ-PCR assays results indicated that c-jun mRNA expression decreased with the rise of c-jun ASODNs concentration. Only 0.125 μmol L-1 c-jun ASODNs could reduce the expression of c-jun gene significantly (p<0.01). And the expression no longer went down when the concentration was up to 1 μmol L-1. Through MTT analysis, it is found that c-junASODNs could inhibit the growth of leydig cells significantly (p<0.01). There is a positive correlation between c-jun ASODNs concentration and growth inhibition ratio of leydig cells (r = 0.8966).
Xiaobo Zheng, Lijuan Qing, Zhiwan Zheng, Jiao Li, Rong Xiao, Libin Wang, Yinzhu Yao, Boqing Wu, Huihao Xu, Maosen Xu, Jing Li, Qun Wu and Liang Ge, 2013. The Effect of C-Jun on Leydig Cells Proliferation of Elemental Status in Rongchang Piglets. Agricultural Journal, 8: 256-261.