Authors : Ping Liu, Xiao Lu Hou, Pei Nie, Yin Hua Han, Yun Fei Huang, Zhen Xing Zoun, Hui Fang Deng, Peng Song, Ming Li and Bao Xiang He
Abstract: Determination of Alanine aminotransferase (ALT) activity plays an important role in the diagnosis of liver function injury. Cows manifest hypernomic fat mobilization in early lactation which causes liver function injury and lead to variation of ALT activity. The study aim was to establish an advanced diagnostic method for determining the activity of ALT and analyze their dynamics characteristics and correlation in milk and blood plasma collected from postpartum dairy cows in early lactation. In this study, 35 healthy Holstein cows were involved. About 187 blood plasma and milk samples were collected from cows 4-10 weeks post-parturition. Samples were collected once weeks in the morning before the cows were feed. In the collected samples, the concentration of ALT was detected by spectrophotometer techniques. The results showed that: ALT activity was significantly lower in milk than that in blood plasma (p<0.05); ALT activity in both blood plasma and milk significantly increased and reached the peak at the same time at week 8 postpartum. According to the results of the study, there was significant positive correlation (r = 0.84, p<0.001) in the concentration of ALT between blood plasma and milk. Therefore, the outcome results suggest that ALT activity determination in milk can replace that in blood plasma in case of detection in the earlier lactation of cows days postpartum. This method may contribute to an extensive survey and monitoring systems.
Ping Liu, Xiao Lu Hou, Pei Nie, Yin Hua Han, Yun Fei Huang, Zhen Xing Zoun, Hui Fang Deng, Peng Song, Ming Li and Bao Xiang He, 2013. Dynamic Monitoring of ALT and Correlation Analysis in Blood Plasma and Milk of Holstein Cows. Agricultural Journal, 8: 51-55.