Abstract: This study was focused on organizational development of district in developing and supervising village governance. It was intended to understand on organizational development of district at Ende Regency, East Nusa Tenggara Province, its supporting and inhibiting factors. Research design used was qualitative with descriptive approach. Data in this study was collected by interviewing respondents (purposive sampling), document study and field observation. Informants in this study were Regent of Ende, Head of DPRD of Ende Regency, Regional Secretary of Ende Regency Assistant Regional Secretary of Ende Regency for Administrative and Public Welfare, Head of Bappeda of Ende Regency, Head of DPPKAD of Ende Regency, Head of BPMPD of Ende Regency, Head of BKD of Ende Regency, Head of Organization and Personnel Division of Regional Secretariat of Ende Regency, Head of General Administration Division of Regional Secretariat of Ende Regency, District Head of East Ende, District Head of Ndona, District Head of Ende, District Head of Kotabaru and District Head of Nangapanda. Result of this study showed that organizational development of district in developing and supervising village governance at Ende Regency was implemented in these phases, changing mindset by focusing on developing performance assessment system; updating by focusing on building individual learning, changing structure by prioritizing the restructuring of work architecture and reempowerment by focusing on changing the regulations through information technology. Main supporting factor of organizational development of district is on the aspect of technology development with effect on the change in social trend. The inhibiting factors deriving from individual is habitual aspect while from organization is group weaknesses. Efforts to overcome the inhibiting factors are by education and communication, encouraging participation, building support and commitment and coercion.