Authors : Dwi Setyorini, Yulian Dwi Utami, Edi Widjayanto, Sri Winarsih, A.S. Noorhamdani and Sumarno Reto Prawiro
Abstract: Shigellosis is a major public health problem, especially in developing countries and the disease caused by Shigella sp. Among four species of Shigella, S. dysenteriae is the most virulent species and is often isolated from cases of Shigellosis. Vaccination is the best effort to eradicate infectious disease that has relation to the Shigellosis. The earlier study reported that protein sub unit pili with Molecular Weight (MW) 49.8 kDa S. dysentriae is the hemagglutinin protein and serves as an adhesion molecule of S. dysenteriae. The aim of this study is to prove the ability of adhesion molecule subunit pili with MW 49.8 kDa S. dysenteriae to increase s-IgA that is protective against S. dysentriae bacterial colonization and protective against the colonic epithelial cells damage. This study was performed by using Balb/c mice divided into 4 group treatments: negative control (no immunization and no S. dysenteriae exposure); positive control (no immunization but the sample was exposed to bacteria); the sample immunized with 12 μg/25 μL ISCOM and exposed to S. dysenteriae and then the sampleMW 49.8 kDa S. dysentriae conjugated with 12 μg/25 μL ISCOM and exposed to S. dysenteriae. Immunizations were given on the day 0, 7 and 28. On the day 35, the mice were killed and the s-IgA concentrations were examined using ELISA. Colon protection was measured with Mice Ligated Ileal Loop (MLIL) Method followed with the counting of colon bacterial colonization by using bacterial colony counter after culturing on Salmonella Shigella Agar (SSA). Histopathology examination was used to identify the magnitude of colonic epithelial cell damage. The results showed that immunization used adhesion molecule subunit pili with MW 49.8 kDa S. dysenteriae conjugated with ISCOM could increase s-IgA concentration, reduce the colonic bacterial colonization and minimize the colon epithelial cells damage in negative control compared to positive controls and ISCOM immunization (p<0.05). It can be concluded, oral administration of S. dysenteriae pili 49.8 kDa conjugated with ISCOM can increase s-IgA protective against bacterial colonization of the colon and colonic epithelial cells damage.
Dwi Setyorini, Yulian Dwi Utami, Edi Widjayanto, Sri Winarsih, A.S. Noorhamdani and Sumarno Reto Prawiro, 2013. Protectivity of Adhesion Molecules Pili 49,8 kDa Shigela dysenteriae Conjugated with ISCOM against Bacterial Colonization and Colonic Epithelial Cells Damage in Mice. International Journal of Tropical Medicine, 8: 19-26.