Authors : Wiyono Hadi, Edi Handoko, A.S. Noorhamdani and Sumarno Reto Prawiro
Abstract: The biofilm in chronic rhinosinusitis due to the infection by bacteria is a difficult problem to overcome. Bacteria in side embedded in biofilms are difficult to treat. CRS with biofilms has recurrent infections, ongoing mucosal inflammation and persistent postoperative symptoms. Propolis is a natural product that has the potential as an anti-bacterial choice. The purpose this study was to determine the effect of propolis on the growth of bacteria Staphylococcus aureus biofilm from isolate chronic patient rhinosinusitis, using CLSM with staining of green fluorescent nucleic acid Syto9. Isolate Staphylococcus aureus taken from swab discharge of meatus medius of chronic rhinosinusitis patients in functional endoscopy of sinus surgery at PHC Hospital Surabaya, Indonesia. Identification of Staphylococcus aureus using medium Mannitol Salt Agar, Gram Staining, Catalase Test and Coagulase Test. Biofilm produced bacterial cell cultured in congo red agar. EEP macerated of alcohol 70% and after that the biofilm formed put in 24 well culture plate 48 h using EEP solution dosages of 0%, 0.2 was %, 0.4%, 0.8%, 2%, 8%, 10% and negative control. Measurement intensity of expression Syto9 and biofilm depth using CLSM magnification 400x. Each well checked for 3x field of view. Ethanolic Extract of Propolis Trigona sp. solution dosages of 0, 0.2, 0.4, 0.8, 2, 8, 10% and negative control, biofilm Staphylococcus aureus obtained decreased intensity of expression Syto9 and biofilm depth. Kruskal Wallis test, Syto9 the result of significance (p) was 0.001. The result showed there was a difference of intensity of expression Syto9 results in the treatment dose group (p<α = 0.05). In the biofilm depth, the result of significance (p) was 0.001. The study showed that there was a difference of biofilm results in treatment dose group (p<α = 0.05). In Post Hoc test the EEP Trigona sp. 2-10% dose can inhibit the growth of biofilms.
Wiyono Hadi, Edi Handoko, A.S. Noorhamdani and Sumarno Reto Prawiro, 2020. Effect of Ethanolic Extract of Propolis Trigona Spp. Malang Indonesia on Isolate Staphylococcus aureus Biofilm Architecture from Chronic Rhinosinusitis: A Confocal Laser Scanning Microscopic Study. International Journal of Tropical Medicine, 15: 97-102.