Authors : T.A. Strauch , J.A. Carroll , T. J. Fangman , C. E. Wiedmeyer and A. K. Hambach
Abstract: The acute phase response of pigs infected with an oral dose of Escherichia coli has not been well defined. To better define this immune response, serum concentrations of cortisol, acute phase proteinsand several cytokines were monitored. Twenty-four crossbred pigs were assigned to blood collection (n=12) or rectal temperature (RT) measurement (n=12) groups. Blood collection pigs were non-surgically cannulated one day prior to blood collection. At 0 h, all pigs received a 10 mL oral dose of 2.4 x 108 colony-forming units of E. coli K88 via a nasogastric tube. Blood collection and rectal temperature monitoring occurred hourly from -1 to 5 h, every 30 minutes from 5 to 8 h and at 24 h post-innoculation. Rectal temperatures and serum concentrations of cortisol (CS), interferon (IFN)-? , interleukin (IL)-1?, IL-6, IL-10, serum amyloid A (SAA), C-reactive protein (CRP) and haptoglobin (HG) increased (P < 0.0007) after E. coli administration. Serum concentrations of IL-4 and IL-8 did not change and tumor necrosis factor (TNF)-? concentrations were undetectable. Positive correlations existed between RT and CS (r = 0.49; P < 0.0001), IFN-? (r = 0.29; P < 0.0009), IL-1? (r = 0.45; P < 0.0001), IL-6 (r = 0.31; P < 0.0003), IL-10 (r = 0.35; P < 0.0001) and CRP (P < 0.0007). These data suggest that circulating TNF-? does not play an integral role in the febrile response in pigs challenged with E. coli; however, CS, IL-1?, IL-6, IL-10 and IFN-? may play an integral role in initiating and controlling the febrile response.
T.A. Strauch , J.A. Carroll , T. J. Fangman , C. E. Wiedmeyer and A. K. Hambach , 2004. The Acute Phase Response of Escherichia coli Challenged Pigs Exhibiting a Febrile Response in Absence of Elevated TNF-. Journal of Animal and Veterinary Advances, 3: 218-227.