Authors : Hongzhuan Wu, Narendra K Singh , Robert Locy , Gunn Karyn Scissum and Joseph J. Giambrone
Abstract: The S1 genes of a pathogenic avian reovirus (S1133) isolate and a highly attenuated vaccine (SynVacR) strain, both from the United States (US), were determined and compared. Genes were amplified with a reverse transcription polymerase chain reaction and cloned into a T-vector. Recombinants were subjected to sequencing and sequences analyzed phylogenetically. They were compared with sequences of other avian reoviruses (ARVs) from the US as well as other countries, that were available from the GeneBank database. Reoviruses could be grouped into two major genetypes and five sub-clusters. A close genetic relationship among ARV isolates from different geographic areas was observed. Pathogenic field isolates from all countries belonged to one group, whereas the SynVacR vaccine strain belonged to a separate group. Results revealed that the genetic diversity in S1 gene could differentiate ARV isolates based on pathogenicity, but not country of origin.
Hongzhuan Wu, Narendra K Singh , Robert Locy , Gunn Karyn Scissum and Joseph J. Giambrone , 2004. Comparison of Avian Reovirus S1 Genes. Journal of Animal and Veterinary Advances, 3: 325-328.