Authors : F.K. Mohammad , G.A.M. Faris , A.S. Alias and B.Kh. Al-Baggou
Abstract: Measurement of blood cholinesterase activity is a useful tool for monitoring exposure of animals to organophosphate and carbamate insecticides. A modified electrometric method was described for measurement of normal reference range values of plasma and erythrocyte cholinesterase activities in cattle, sheep and goats of both sexes. The reaction mixture contained 3 mL distilled water, 3 mL barbital-phosphate buffer (pH 8.1), 0.2 mL plasma or erythrocytes and 0.1 mL acetylthiocholine iodide (7.5%) as a substrate. The mixture was incubated at 37?C for 20 min in cattle, 30 min in sheep and 40 min in goats. The pH of the reaction mixture was determined by a pH meter before and after the incubation. The initial pH was measured before the substrate addition. The enzyme activity was expressed as ? pH/incubation time. The mean normal reference range values of plasma cholinesterase activity (? pH/incubation time) in males and females of the three animal species were as follows, respectively: cattle (0.10 and 0.17), sheep (0.21 and 0.19) and goats (0.22 and 0.22), whereas those of the erythrocyte cholinesterase activity (? pH/incubation time) were as follows, respectively: cattle (0.91 and 0.89), sheep (0.63 and 0.62) and goats (0.54 and 0.44). The study establishes for the first time by using the described electrometric method normal reference range values of blood cholinesterase activities in cattle, sheep and goats.
F.K. Mohammad , G.A.M. Faris , A.S. Alias and B.Kh. Al-Baggou , 2005. Blood Cholinesterase Activities in Cattle, Sheep andGoats Measured by a Modified Electrometric Method . Journal of Animal and Veterinary Advances, 4: 923-926.